four-parameter curve-fitting on a dose–response curve Search Results


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four-parameter logistic regression dose-response curves  (GraphPad Software Inc)
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Impact of 1 and 2 compounds on BT474 breast cancer cells. ( A ) To assess whether 1 and 2 could trigger cytotoxic effects on living cells, BT474 cells were treated with serial dilutions of 1 and 2 (or sterile DMSO). After three days, live cells were detected by standard MTT assay, highlighting the appearance of cytotoxic effects above 75 μM for compound 1 . Compound 2 did not show cytotoxic effects even at 250 μM. ***p < 0.001; ****p < 0.0001. ( B ) 1 and 2 compounds inhibit cell proliferation of BT474 and ( C ) SKBR3 breast cancer cell lines. Cells were treated with different concentration of the 1 and 2 and proliferation was assayed three days later by performing MTT assay. Results were fitted with four-parameter dose-response curve using GraphPad <t>Prism7</t> software. ( D ) EC50 values for 1 and 2 in BT474 and SKBR3 breast cancer cell lines are represented as histogram with 95% confidence intervals. ( E ) SKBR3 cells were treated with Trastuzumab (25 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses. After three days, live cells were detected by standard MTT assay. Data are represented as mean ± SD. *p < 0.05, **p < 0.01. ( D ) SKH100 Trastuzumab-resistant cells were treated with Trastuzumab (100 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses. After three days, live cells were detected by standard MTT assay. Data are represented as mean ± SD. *p < 0.05, **p < 0.01.
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Impact of 1 and 2 compounds on BT474 breast cancer cells. ( A ) To assess whether 1 and 2 could trigger cytotoxic effects on living cells, BT474 cells were treated with serial dilutions of 1 and 2 (or sterile DMSO). After three days, live cells were detected by standard MTT assay, highlighting the appearance of cytotoxic effects above 75 μM for compound 1 . Compound 2 did not show cytotoxic effects even at 250 μM. ***p < 0.001; ****p < 0.0001. ( B ) 1 and 2 compounds inhibit cell proliferation of BT474 and ( C ) SKBR3 breast cancer cell lines. Cells were treated with different concentration of the 1 and 2 and proliferation was assayed three days later by performing MTT assay. Results were fitted with four-parameter dose-response curve using GraphPad Prism7 software. ( D ) EC50 values for 1 and 2 in BT474 and SKBR3 breast cancer cell lines are represented as histogram with 95% confidence intervals. ( E ) SKBR3 cells were treated with Trastuzumab (25 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses. After three days, live cells were detected by standard MTT assay. Data are represented as mean ± SD. *p < 0.05, **p < 0.01. ( D ) SKH100 Trastuzumab-resistant cells were treated with Trastuzumab (100 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses. After three days, live cells were detected by standard MTT assay. Data are represented as mean ± SD. *p < 0.05, **p < 0.01.

Journal: Scientific Reports

Article Title: Modeling ErbB2-p130Cas interaction to design new potential anticancer agents

doi: 10.1038/s41598-019-39510-w

Figure Lengend Snippet: Impact of 1 and 2 compounds on BT474 breast cancer cells. ( A ) To assess whether 1 and 2 could trigger cytotoxic effects on living cells, BT474 cells were treated with serial dilutions of 1 and 2 (or sterile DMSO). After three days, live cells were detected by standard MTT assay, highlighting the appearance of cytotoxic effects above 75 μM for compound 1 . Compound 2 did not show cytotoxic effects even at 250 μM. ***p < 0.001; ****p < 0.0001. ( B ) 1 and 2 compounds inhibit cell proliferation of BT474 and ( C ) SKBR3 breast cancer cell lines. Cells were treated with different concentration of the 1 and 2 and proliferation was assayed three days later by performing MTT assay. Results were fitted with four-parameter dose-response curve using GraphPad Prism7 software. ( D ) EC50 values for 1 and 2 in BT474 and SKBR3 breast cancer cell lines are represented as histogram with 95% confidence intervals. ( E ) SKBR3 cells were treated with Trastuzumab (25 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses. After three days, live cells were detected by standard MTT assay. Data are represented as mean ± SD. *p < 0.05, **p < 0.01. ( D ) SKH100 Trastuzumab-resistant cells were treated with Trastuzumab (100 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses. After three days, live cells were detected by standard MTT assay. Data are represented as mean ± SD. *p < 0.05, **p < 0.01.

Article Snippet: Results were fitted with four-parameter dose-response curve using GraphPad Prism7 software. ( D ) EC50 values for 1 and 2 in BT474 and SKBR3 breast cancer cell lines are represented as histogram with 95% confidence intervals. ( E ) SKBR3 cells were treated with Trastuzumab (25 μg/ml) either alone or in combination with compound 1 or 2 at the indicated doses.

Techniques: MTT Assay, Concentration Assay, Software